Amino Acid Analyzers

Amino Acid Analyzers

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The ARACUS is a dedicated amino acid analyser for high end analysis of amino acids in research, food quality control (own patent 10 2016 010 887), beverages and feedstuff and in the clinical laboratory. It combines innovative technologies with the classic routine analysis of amino acids by post-column derivatization with ninhydrin and the detection at 440 nm and 570 nm using maintenance-free LED photometers. The setup of the instrument allows the continuous analysis of 96 samples (2 x 48 vials) in a cooled autosampler. The pump allows the simultaneous operation of two fluid lines (eluent line and reactive line), which guarantees a constant mixing. Eluents and reagent solution are selectively chosen through valves and are ideally mixed to each other. The addition of ninhydrin provides a quantitative analysis statement. A washing routine of the injection valve and syringe pump avoids cross contaminations.The level of the individual reagents can be monitored during the runs and warns the user if the quantity is no longer sufficient.

The data acquisition and analysis software aminoPeak or Clarity records two analogue channels simultaneously (570 nm, 440 nm). Chromatograms are shown online. Internal databases allow fast peak identification. Quantitative calculations are performed with internal or external standards, dilution and/or multiplication factors. Several analysis runs can be compared using compiler programmes. The results of each analysis are documented in an individual report.

Ready-to-use kits

The concept behind the ready-to-use eluent and reagent kits allows the reproducible analysis of amino acid samples. Each kit is produced under standardised procedures, tested and certified before shipping. Depending on the application, the eluent and reagent kit contains the necessary amount for about 500 runs. Kits and columns are optiomally matched to each other.

Technical Specifications:

  • method:

          post colum derivatization with ninhydrine

  • spectroscopic detection:

          LED photometers: 440 nm + 570 nm

  • detection limit:

          < 2 nmol/mL

  • reproducibility of retention time:

          < 0.3 % RSD

  • reproducibility of area:

           ca. 1 % RSD

ARACUS advantages

  • detectors with maintenance-free LED technology (440 & 570 nm)/ no UV/VIS lamp degeneration and lamp exchange
  • chloride-free and citrate-free buffer system / less aggressive to the components of the liquid handling tubes, fittings and connection
  • small eluent consumption / about 200 µL/min in analysis mode
  • high sensitivity of amino acids / choice of two sensitivity detector levels
  • level sensors for eluents / warn the user if needed fluidic volume is too low for planned analysis
  • easy access to all components of the entire system
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OPTIONS


ARACUS advanced

2 pump system allows to operate with a lower noise level and are required for OPA post column derivatization method.

OPA method

Sensitive fluorescent detection reagent for amines with o-phtalaldehyde (OPA). Configuration ARACUS advanced is needed.

Level control

Active control of fluid volume by weight cell of each bottle

IQ and OQ documents

prepared documents for the IQ and OQ procedure. Validation support can also be requested additionally. 

Inert gas

It supplies the main unit and eluent box with a protective gas atmosphere by reducing the presence of atmospheric oxygen to enhance the signal to noise ratio.

Software 21 CFR part 11 compliance

As an option, Clarity can be used if 21 CFR Part 11 conformity is required for manual and automatoc peak integration with integreated IQ/OQ procedure for software.

InVitro diagnostic Kit according to DIN ISO 13485

IVD kits for the qualitative and quantitative determination of amino acids in physiological fluids, for in vitro diagnostic use with ARACUS amino acid analyser.